6Y2K

Crystal structure of beta-galactosidase from the psychrophilic Marinomonas ef1

Summary for 6Y2K

• Entry DOI: 10.2210/pdb6y2k/pdb
• Descriptor: beta-galactosidase, CHLORIDE ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: glycosyl hydrolase, psychrophilic enzyme, hexameric structure, cold adaptation, enzyme kinetics, cooperativity, hydrolase
• Biological source: Marinomonas sp. ef1
• Total number of polymer chains: 1
• Total formula weight: 76240.78

Authors

Mangiagalli, M.,Lapi, M.,Maione, S.,Orlando, M.,Brocca, S.,Pesce, A.,Barbiroli, A.,Pucciarelli, S.,Camilloni, C.,Lotti, M. (deposition date: 2020-02-16, release date: 2020-05-20, Last modification date: 2024-01-24)

• Primary citation: Mangiagalli, M.,Lapi, M.,Maione, S.,Orlando, M.,Brocca, S.,Pesce, A.,Barbiroli, A.,Camilloni, C.,Pucciarelli, S.,Lotti, M.,Nardini, M.; The co-existence of cold activity and thermal stability in an Antarctic GH42 beta-galactosidase relies on its hexameric quaternary arrangement.; Febs J., 288:546-565, 2021; PubMed: 32363751; DOI: 10.1111/febs.15354

Experimental method

X-RAY DIFFRACTION (1.9 Å)