6Y2K
Crystal structure of beta-galactosidase from the psychrophilic Marinomonas ef1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 133.332, 133.332, 232.574 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 116.290 - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kwg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.249 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.290 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.255 | 0.674 |
| Number of reflections | 96335 | 3405 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 29.4 | 33.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 0.5 M NaCl, 100 mM Na-citrate, 2% ethylene imine polymer. |
