6XXW

Structure of beta-D-Glucuronidase for Dictyoglomus thermophilum.

6XXW の概要

• エントリーDOI: 10.2210/pdb6xxw/pdb
• 分子名称: Beta-glucuronidase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
• 機能のキーワード: glycoside hydrolase, glucuronide, hydrolase
• 由来する生物種: Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71197.18

構造登録者

Lafite, P.,Daniellou, R. (登録日: 2020-01-28, 公開日: 2020-07-15, 最終更新日: 2024-01-24)

主引用文献

Kurdziel, M.,Kopec, M.,Paris, A.,Lewinski, K.,Lafite, P.,Daniellou, R.
Thioglycoligation of aromatic thiols using a natural glucuronide donor.
Org.Biomol.Chem., 18:5582-5585, 2020

PubMed Abstract: The β-d-glucuronidase DtGlcA from Dictyoglomus thermophilum was engineered to generate an active thioglycoligase that is able to catalyse the formation of numerous S-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors for S-glycosylation. Noteworthily, the DtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used synthetic para-nitrophenyl glycosides.

PubMed: 32671369
DOI: 10.1039/d0ob00226g

実験手法

X-RAY DIFFRACTION (1.851 Å)