6XP9

STRUCTURE OF HUMAN PREGNANE X RECEPTOR LIGAND BINDING DOMAIN BOUND TETHERED WITH SRC co-activator peptide IN COMPLEX WITH (S,S)-1

6XP9 の概要

• エントリーDOI: 10.2210/pdb6xp9/pdb
• 分子名称: Nuclear receptor subfamily 1 group I member 2,Nuclear receptor coactivator 1 fusion, (2S)-tert-butoxy[7-(8-fluoro-5-methyl-3,4-dihydro-2H-1-benzopyran-6-yl)-5-methyl-2-phenylpyrazolo[1,5-a]pyrimidin-6-yl]acetic acid, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: nuclear receptor, multiple binding modes, xenobiotic, promiscuous, ligand, nuclear hormone receptor, nuclear protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 80890.49

構造登録者

Khan, J.A. (登録日: 2020-07-08, 公開日: 2020-09-23, 最終更新日: 2023-10-18)

主引用文献

Sivaprakasam, P.,Wang, Z.,Meanwell, N.A.,Khan, J.A.,Langley, D.R.,Johnson, S.R.,Li, G.,Pendri, A.,Connolly, T.P.,Gao, M.,Camac, D.M.,Klakouski, C.,Zvyaga, T.,Cianci, C.,McAuliffe, B.,Ding, B.,Discotto, L.,Krystal, M.R.,Jenkins, S.,Peese, K.M.,Narasimhulu Naidu, B.
Structure-based amelioration of PXR transactivation in a novel series of macrocyclic allosteric inhibitors of HIV-1 integrase.
Bioorg.Med.Chem.Lett., 30:127531-127531, 2020

PubMed Abstract: Previous studies have identified a series of imidazo[1,2-a]pyridine (IZP) derivatives as potent allosteric inhibitors of HIV-1 integrase (ALLINIs) and virus infection in cell culture. However, IZPs were also found to be relatively potent activators of the pregnane-X receptor (PXR), raising the specter of induction of CYP-mediated drug disposition pathways. In an attempt to modify PXR activity without affecting anti-HIV-1 activity, rational structure-based design and modeling approaches were used. An X-ray cocrystal structure of (S,S)-1 in the PXR ligand binding domain (LBD) allowed an examination of the potential of rational structural modifications designed to abrogate PXR. The introduction of bulky basic amines at the C-8 position provided macrocyclic IZP derivatives that displayed potent HIV-1 inhibitory activity in cell culture with no detectable PXR transactivation at the highest concentration tested.

PubMed: 32890685
DOI: 10.1016/j.bmcl.2020.127531

実験手法

X-RAY DIFFRACTION (2.27 Å)