6XKL

SARS-CoV-2 HexaPro S One RBD up

6XKL の概要

• エントリーDOI: 10.2210/pdb6xkl/pdb
• EMDBエントリー: 22221 22222
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: sars-cov-2, glycoprotein, fusion protein, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 438144.55

構造登録者

Wrapp, D.,Hsieh, C.-L.,Goldsmith, J.A.,McLellan, J.S. (登録日: 2020-06-26, 公開日: 2020-07-15, 最終更新日: 2024-10-23)

主引用文献

Hsieh, C.L.,Goldsmith, J.A.,Schaub, J.M.,DiVenere, A.M.,Kuo, H.C.,Javanmardi, K.,Le, K.C.,Wrapp, D.,Lee, A.G.,Liu, Y.,Chou, C.W.,Byrne, P.O.,Hjorth, C.K.,Johnson, N.V.,Ludes-Meyers, J.,Nguyen, A.W.,Park, J.,Wang, N.,Amengor, D.,Lavinder, J.J.,Ippolito, G.C.,Maynard, J.A.,Finkelstein, I.J.,McLellan, J.S.
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes.
Science, 369:1501-1505, 2020

PubMed Abstract: The coronavirus disease 2019 (COVID-19) pandemic has led to accelerated efforts to develop therapeutics and vaccines. A key target of these efforts is the spike (S) protein, which is metastable and difficult to produce recombinantly. We characterized 100 structure-guided spike designs and identified 26 individual substitutions that increased protein yields and stability. Testing combinations of beneficial substitutions resulted in the identification of HexaPro, a variant with six beneficial proline substitutions exhibiting higher expression than its parental construct (by a factor of 10) as well as the ability to withstand heat stress, storage at room temperature, and three freeze-thaw cycles. A cryo-electron microscopy structure of HexaPro at a resolution of 3.2 angstroms confirmed that it retains the prefusion spike conformation. High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

PubMed: 32703906
DOI: 10.1126/science.abd0826

実験手法

ELECTRON MICROSCOPY (3.21 Å)