6XF5

Cryo-EM structure of a biotinylated SARS-CoV-2 spike probe in the prefusion state (RBDs down)

Summary for 6XF5

• Entry DOI: 10.2210/pdb6xf5/pdb
• EMDB information: 22161
• Descriptor: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: fusion protein, molecular probe, spike glycoprotein, covid-19, rbd, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• Total number of polymer chains: 3
• Total formula weight: 429226.19

Authors

Cerutti, G.,Gorman, J.,Kwong, P.D.,Shapiro, L. (deposition date: 2020-06-15, release date: 2020-09-02, Last modification date: 2020-12-02)

• Primary citation: Zhou, T.,Teng, I.T.,Olia, A.S.,Cerutti, G.,Gorman, J.,Nazzari, A.,Shi, W.,Tsybovsky, Y.,Wang, L.,Wang, S.,Zhang, B.,Zhang, Y.,Katsamba, P.S.,Petrova, Y.,Banach, B.B.,Fahad, A.S.,Liu, L.,Acevedo, S.N.L.,Madan, B.,de Souza, M.O.,Pan, X.,Wang, P.,Wolfe, J.R.,Yin, M.,Ho, D.D.,Phung, E.,DiPiazza, A.,Chang, L.,Abiona, O.,Corbett, K.S.,DeKosky, B.J.,Graham, B.S.,Mascola, J.R.,Misasi, J.,Ruckwardt, T.,Sullivan, N.J.,Shapiro, L.,Kwong, P.D.; Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.; SSRN, :3639618-3639618, 2020; PubMed: 32742241; DOI: 10.2139/ssrn.3639618

Experimental method

ELECTRON MICROSCOPY (3.45 Å)