6XE6

Structure of Human Dispatched-1 (DISP1)

Summary for 6XE6

• Entry DOI: 10.2210/pdb6xe6/pdb
• EMDB information: 22144
• Descriptor: Protein dispatched homolog 1, CHOLESTEROL HEMISUCCINATE, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: hedgehog, secretion, sterol binding, sterol-sensing domain, membrane protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 127153.59

Authors

Chen, H.,Liu, Y.,Li, X. (deposition date: 2020-06-12, release date: 2020-07-08, Last modification date: 2021-01-20)

Primary citation

Chen, H.,Liu, Y.,Li, X.
Structure of human Dispatched-1 provides insights into Hedgehog ligand biogenesis.
Life Sci Alliance, 3:-, 2020

PubMed Abstract: Hedgehog (HH) signaling is essential for metazoan development. The HH ligand is secreted into the extracellular space by a cell surface protein named Dispatched-1 (DISP1). Here, we report the cryo-EM structure of human DISP1 protein. DISP1 contains 12 transmembrane helices (TMs) and two extracellular domains (ECDs). Its ECDs reveal an open state, in contrast to its structural homologues PTCH1 and NPC1, whose extracellular/luminal domains adopt a closed state. The low-resolution structure of the DISP1 complex with dual lipid-modified HH ligand reveals how the ECDs of DISP1 engage with HH ligand. Moreover, several cholesterol-like molecules are found in the TMs, implying a transport-like function of DISP1.

PubMed: 32646883
DOI: 10.26508/lsa.202000776

Experimental method

ELECTRON MICROSCOPY (4.54 Å)