6XB3

Structure of AcNPV poxin in post-reactive state with Gp[2'-5']Ap[3']

6XB3 の概要

• エントリーDOI: 10.2210/pdb6xb3/pdb
• 分子名称: Poxin, 2',5'-GpAp (3 entities in total)
• 機能のキーワード: poxin, p26, baculovirus, nucleopolyhedrovirus, innate immunity, nuclease, cgas, cgamp, sting, hydrolase
• 由来する生物種: Autographa californica nuclear polyhedrosis virus (AcMNPV)
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 449470.70

構造登録者

Eaglesham, J.B.,McCarty, K.L.,Kranzusch, P.J. (登録日: 2020-06-05, 公開日: 2020-11-25, 最終更新日: 2024-04-03)

主引用文献

Eaglesham, J.B.,McCarty, K.L.,Kranzusch, P.J.
Structures of diverse poxin cGAMP nucleases reveal a widespread role for cGAS-STING evasion in host-pathogen conflict.
Elife, 9:-, 2020

PubMed Abstract: DNA viruses in the family encode poxin enzymes that degrade the immune second messenger 2'3'-cGAMP to inhibit cGAS-STING immunity in mammalian cells. The closest homologs of poxin exist in the genomes of insect viruses suggesting a key mechanism of cGAS-STING evasion may have evolved outside of mammalian biology. Here we use a biochemical and structural approach to discover a broad family of 369 poxins encoded in diverse viral and animal genomes and define a prominent role for 2'3'-cGAMP cleavage in metazoan host-pathogen conflict. Structures of insect poxins reveal unexpected homology to flavivirus proteases and enable identification of functional self-cleaving poxins in RNA-virus polyproteins. Our data suggest widespread 2'3'-cGAMP signaling in insect antiviral immunity and explain how a family of cGAS-STING evasion enzymes evolved from viral proteases through gain of secondary nuclease activity. Poxin acquisition by poxviruses demonstrates the importance of environmental connections in shaping evolution of mammalian pathogens.

PubMed: 33191912
DOI: 10.7554/eLife.59753

実験手法

X-RAY DIFFRACTION (1.9 Å)