6X65

Legionella pneumophila Dot/Icm T4SS

This is a non-PDB format compatible entry.

Summary for 6X65

• Entry DOI: 10.2210/pdb6x65/pdb
• EMDB information: 22070
• Descriptor: Type IV secretion system unknown protein fragment, DotC, DotD, ... (6 entities in total)
• Functional Keywords: secretion, t4ss, dot, protein transport
• Biological source: Legionella pneumophila; More
• Total number of polymer chains: 153
• Total formula weight: 3504416.28

Authors

Durie, C.L.,Sheedlo, M.J.,Chung, J.M.,Byrne, B.G.,Su, M.,Knight, T.,Swanson, M.S.,Lacy, D.B.,Ohi, M.D. (deposition date: 2020-05-27, release date: 2020-10-07, Last modification date: 2024-03-06)

Primary citation

Durie, C.L.,Sheedlo, M.J.,Chung, J.M.,Byrne, B.G.,Su, M.,Knight, T.,Swanson, M.,Lacy, D.B.,Ohi, M.D.
Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex.
Elife, 9:-, 2020

PubMed Abstract: is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.

PubMed: 32876045
DOI: 10.7554/eLife.59530

Experimental method

ELECTRON MICROSCOPY (3.7 Å)