6X0O

Single-Particle Cryo-EM Structure of Arabinosyltransferase EmbB from Mycobacterium smegmatis

Summary for 6X0O

• Entry DOI: 10.2210/pdb6x0o/pdb
• EMDB information: 21983
• Descriptor: Integral membrane indolylacetylinositol arabinosyltransferase EmbB, CALCIUM ION, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE (3 entities in total)
• Functional Keywords: glycosyltransferase, membrane protein, nanodisc
• Biological source: Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
• Total number of polymer chains: 1
• Total formula weight: 120361.47

Authors

Tan, Y.Z.,Rodrigues, J.,Keener, J.E.,Zheng, R.B.,Brunton, R.,Kloss, B.,Giacometti, S.I.,Rosario, A.L.,Zhang, L.,Niederweis, M.,Clarke, O.B.,Lowary, T.L.,Marty, M.T.,Archer, M.,Potter, C.S.,Carragher, B.,Mancia, F. (deposition date: 2020-05-17, release date: 2020-06-10, Last modification date: 2024-03-06)

Primary citation

Tan, Y.Z.,Rodrigues, J.,Keener, J.E.,Zheng, R.B.,Brunton, R.,Kloss, B.,Giacometti, S.I.,Rosario, A.L.,Zhang, L.,Niederweis, M.,Clarke, O.B.,Lowary, T.L.,Marty, M.T.,Archer, M.,Potter, C.S.,Carragher, B.,Mancia, F.
Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.
Nat Commun, 11:3396-3396, 2020

PubMed Abstract: Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.

PubMed: 32636380
DOI: 10.1038/s41467-020-17202-8

Experimental method

ELECTRON MICROSCOPY (3.3 Å)