6WU0

CryoEM structure of Burkholderia pseudomallei hopanoid biosynthesis-associated RND transporter HpnN

Summary for 6WU0

• Entry DOI: 10.2210/pdb6wu0/pdb
• EMDB information: 21901
• Descriptor: Hopanoid biosynthesis associated RND transporter like protein HpnN (1 entity in total)
• Functional Keywords: cryoem structure, hopanoid transporter hpnn, protein transport
• Biological source: Burkholderia pseudomallei MSHR346
• Total number of polymer chains: 1
• Total formula weight: 92600.93

Authors

Lyu, M.,Yu, E.W. (deposition date: 2020-05-04, release date: 2021-02-03, Last modification date: 2025-05-28)

Primary citation

Su, C.C.,Lyu, M.,Morgan, C.E.,Bolla, J.R.,Robinson, C.V.,Yu, E.W.
A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins.
Nat.Methods, 18:69-75, 2021

PubMed Abstract: Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of different sizes and dimensions, from a heterogeneous, impure protein sample. We also use the BaR methodology to elucidate structural information from Escherichia coli K12 crude membrane and raw lysate. The findings demonstrate that it is possible to solve high-resolution structures of a number of relatively small (<100 kDa) and less abundant (<10%) unidentified membrane proteins within a single, heterogeneous sample. Importantly, these results highlight the potential of cryo-EM for systems structural proteomics.

PubMed: 33408407
DOI: 10.1038/s41592-020-01021-2

Experimental method

ELECTRON MICROSCOPY (3.59 Å)