6WTY

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 253245

6WTY の概要

• エントリーDOI: 10.2210/pdb6wty/pdb
• 関連するPDBエントリー: 6WM9 6WN1 6WNO 6WOZ 6WQO 6WTU 6WTV
• 分子名称: reticulocyte binding protein 2b, 253245 Fab light chain, 253245 Fab heavy chain (3 entities in total)
• 機能のキーワード: invasion, plasmodium vivax, malaria, antibody complex, cell invasion
• 由来する生物種: Plasmodium vivax (strain Salvador I); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 342638.24

構造登録者

Chan, L.J.,Dietrich, M.H.,Tham, W.H. (登録日: 2020-05-04, 公開日: 2021-01-27, 最終更新日: 2024-10-23)

主引用文献

Chan, L.J.,Gandhirajan, A.,Carias, L.L.,Dietrich, M.H.,Vadas, O.,Visentin, R.,Franca, C.T.,Menant, S.,Soldati-Favre, D.,Mueller, I.,King, C.L.,Tham, W.H.
Naturally acquired blocking human monoclonal antibodies to Plasmodium vivax reticulocyte binding protein 2b.
Nat Commun, 12:1538-1538, 2021

PubMed Abstract: Plasmodium vivax preferentially invades reticulocytes and recognition of these cells is mediated by P. vivax Reticulocyte Binding Protein 2b (PvRBP2b) binding to human Transferrin receptor 1 (TfR1) and Transferrin (Tf). Longitudinal cohort studies in Papua New Guinea, Thailand and Brazil show that PvRBP2b antibodies are correlated with protection against P. vivax infection and disease. Here, we isolate and characterize anti-PvRBP2b human monoclonal antibodies from two individuals in Cambodia with natural P. vivax infection. These antibodies bind with high affinities and map to different regions of PvRBP2b. Several human antibodies block PvRBP2b binding to reticulocytes and inhibit complex formation with human TfR1-Tf. We describe different structural mechanisms for functional inhibition, including either steric hindrance with TfR1-Tf or the reticulocyte membrane. These results show that naturally acquired human antibodies against PvRBP2b can inhibit its function which is important for P. vivax invasion.

PubMed: 33750786
DOI: 10.1038/s41467-021-21811-2

実験手法

X-RAY DIFFRACTION (3.481 Å)