6WTY
Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 253245
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.559, 78.189, 312.080 |
Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
Resolution | 48.701 - 3.481 |
R-factor | 0.2666 |
Rwork | 0.262 |
R-free | 0.30110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w53 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.795 | 48.795 | 3.690 |
High resolution limit [Å] | 3.480 | 10.240 | 3.480 |
Rmerge | 0.772 | 0.125 | 2.856 |
Rmeas | 0.857 | 0.139 | 3.179 |
Total number of observations | 234345 | ||
Number of reflections | 43497 | 1809 | 6551 |
<I/σ(I)> | 2.54 | 10.9 | 0.53 |
Completeness [%] | 98.7 | 98.5 | 93.1 |
Redundancy | 5.388 | 5.132 | 5.059 |
CC(1/2) | 0.850 | 0.987 | 0.131 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 0.2 M sodium dihydrogen phosphate pH 4.0, 16% (w/v) PEG 3,350 |