6WNW
Active 70S ribosome without free 5S rRNA and bound with A- and P- tRNA
This is a non-PDB format compatible entry.
Summary for 6WNW
| Entry DOI | 10.2210/pdb6wnw/pdb |
| EMDB information | 21856 21857 21858 |
| Descriptor | 50S ribosomal protein L2, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (57 entities in total) |
| Functional Keywords | ribosome, 23s-cp5s rrna, engineering |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 57 |
| Total formula weight | 2220702.12 |
| Authors | Loveland, A.B.,Korostelev, A.A.,Mankin, A.S.,Huang, S.,Aleksashin, N.A.,Klepacki, D.,Reier, K.,Kefi, A.,Szal, A.,Remme, J.,Jaeger, L.,Vazquez-Laslop, N. (deposition date: 2020-04-23, release date: 2020-06-24, Last modification date: 2024-03-06) |
| Primary citation | Huang, S.,Aleksashin, N.A.,Loveland, A.B.,Klepacki, D.,Reier, K.,Kefi, A.,Szal, T.,Remme, J.,Jaeger, L.,Vazquez-Laslop, N.,Korostelev, A.A.,Mankin, A.S. Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Nat Commun, 11:2900-2900, 2020 Cited by PubMed Abstract: 5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell survival. By linking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain devoid of free 5S rRNA. Viability of the engineered cells demonstrates that autonomous 5S rRNA is dispensable for cell growth under standard conditions and is unlikely to have essential functions outside the ribosome. The fully assembled ribosomes carrying 23S-5S rRNA are highly active in translation. However, the engineered cells accumulate aberrant 50S subunits unable to form stable 70S ribosomes. Cryo-EM analysis revealed a malformed peptidyl transferase center in the misassembled 50S subunits. Our results argue that the autonomy of 5S rRNA is preserved due to its role in ribosome biogenesis. PubMed: 32518240DOI: 10.1038/s41467-020-16694-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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