6WDT

Enterovirus D68 in complex with human monoclonal antibody EV68-228

Summary for 6WDT

• Entry DOI: 10.2210/pdb6wdt/pdb
• EMDB information: 21647 21648
• Descriptor: viral protein 1, viral protein 2, viral protein 3, ... (6 entities in total)
• Functional Keywords: virus, enterovirus, antibody, structural genomics, center for structural genomics of infectious diseases, csgid, virus-immune system complex, virus/immune system
• Biological source: Enterovirus D68; More
• Total number of polymer chains: 6
• Total formula weight: 120329.46

Authors

Fu, J.,Vogt, M.R.,Klose, T.,Crowe, J.E.,Rossmann, M.G.,Kuhn, R.J.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2020-04-01, release date: 2020-07-15, Last modification date: 2024-11-13)

Primary citation

Vogt, M.R.,Fu, J.,Kose, N.,Williamson, L.E.,Bombardi, R.,Setliff, I.,Georgiev, I.S.,Klose, T.,Rossmann, M.G.,Bochkov, Y.A.,Gern, J.E.,Kuhn, R.J.,Crowe Jr., J.E.
Human antibodies neutralize enterovirus D68 and protect against infection and paralytic disease.
Sci Immunol, 5:-, 2020

PubMed Abstract: Enterovirus D68 (EV-D68) causes outbreaks of respiratory illness, and there is increasing evidence that it causes outbreaks of acute flaccid myelitis (AFM). There are no licensed therapies to prevent or treat EV-D68 infection or AFM disease. We isolated a panel of EV-D68-reactive human monoclonal antibodies that recognize diverse antigenic variants from participants with prior infection. One potently neutralizing cross-reactive antibody, EV68-228, protected mice from respiratory and neurologic disease when given either before or after infection. Cryo-electron microscopy studies revealed that EV68-228 and another potently neutralizing antibody (EV68-159) bound around the fivefold or threefold axes of symmetry on virion particles, respectively. The structures suggest diverse mechanisms of action by these antibodies. The high potency and effectiveness observed in vivo suggest that antibodies are a mechanistic correlate of protection against AFM disease and are candidates for clinical use in humans with EV-D68 infection.

PubMed: 32620559
DOI: 10.1126/sciimmunol.aba4902

Experimental method

ELECTRON MICROSCOPY (3.1 Å)