6WA8

Crystal structure of the E. coli transcription termination factor Rho

6WA8 の概要

• エントリーDOI: 10.2210/pdb6wa8/pdb
• 分子名称: Transcription termination factor Rho, ADENOSINE-5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: atpase, transcription, hydrolase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 285464.09

構造登録者

Fan, C.,Rees, D.C. (登録日: 2020-03-24, 公開日: 2020-09-16, 最終更新日: 2023-10-18)

主引用文献

Fan, C.,Rees, D.C.
Crystal structure of the Escherichia coli transcription termination factor Rho.
Acta Crystallogr.,Sect.F, 76:398-405, 2020

PubMed Abstract: During the crystal structure analysis of an ATP-binding cassette (ABC) transporter overexpressed in Escherichia coli, a contaminant protein was crystallized. The identity of the contaminant was revealed by mass spectrometry to be the Escherichia coli transcription terminator factor Rho, structures of which had been previously determined in different conformational states. Although Rho was present at only ∼1% of the target protein (a bacterial homolog of the eukaryotic ABC transporter of mitochondria from Novosphingobium aromaticivorans; NaAtm1), it preferentially crystallized in space group C2 as thin plates that diffracted to 3.30 Å resolution. The structure of Rho in this crystal form exhibits a hexameric open-ring staircase conformation with bound ATP; this characteristic structure was also observed on electron-microscopy grids of the NaAtm1 preparation.

PubMed: 32880587
DOI: 10.1107/S2053230X20010572

実験手法

X-RAY DIFFRACTION (3.3 Å)