6W3M

Solution NMR Structure of 5'UTR Stem Loop B in DENV4 Flavivirus.

Summary for 6W3M

• Entry DOI: 10.2210/pdb6w3m/pdb
• NMR Information: BMRB: 28094
• Descriptor: RNA (41-MER) (1 entity in total)
• Functional Keywords: rna, 5'utr, structural genomics, seattle structural genomics center for infectious disease, ssgcid
• Biological source: Dengue virus 4 Philippines/H241/1956
• Total number of polymer chains: 1
• Total formula weight: 13254.92

Authors

Sharma, S.,Varani, G.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2020-03-09, release date: 2020-09-23, Last modification date: 2024-05-15)

Primary citation

Sharma, S.,Varani, G.
NMR structure of Dengue West Nile viruses stem-loop B: A key cis-acting element for flavivirus replication.
Biochem.Biophys.Res.Commun., 531:522-527, 2020

PubMed Abstract: Flaviviruses are major emerging human pathogenic viruses that pose a persistent and growing menace to global health. They are enveloped single-stranded RNA viruses with positive polarity transmitted by arthropod vectors like mosquitoes or ticks, responsible for a significant and growing number of human deaths and illnesses. The 5'- and 3'-untranslated regions (UTRs) are highly structured and contain conserved cis-acting RNA elements that participate in viral translation, replication and host adaptation. Despite their role in fiaviviruses replication, few high-resolution structural studies have investigated the RNA elements required for viral replication. Here we report the NMR structures of stem-loop B from WNV and DENV4 viruses. Because this element is required for cyclization of the genome and the activity of the replicative viral enzymes, viral replication rates are sensitive to even small changes in these RNAs. Therefore, this work provides structural insight into a new drug target to reduce flavivirus replication rates.

PubMed: 32807496
DOI: 10.1016/j.bbrc.2020.07.115

Experimental method

SOLUTION NMR