6W32
Crystal structure of Sfh5
Summary for 6W32
Entry DOI | 10.2210/pdb6w32/pdb |
Descriptor | Phosphatidylinositol transfer protein SFH5, PROTOPORPHYRIN IX CONTAINING FE (2 entities in total) |
Functional Keywords | signaling protein |
Biological source | Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) |
Total number of polymer chains | 3 |
Total formula weight | 109032.50 |
Authors | Gulten, G.,Khan, D.,Aggarwal, A.,Krieger, I.,Sacchettini, J.C.,Bankaitis, V.A. (deposition date: 2020-03-08, release date: 2020-11-25, Last modification date: 2024-03-06) |
Primary citation | Khan, D.,Lee, D.,Gulten, G.,Aggarwal, A.,Wofford, J.,Krieger, I.,Tripathi, A.,Patrick, J.W.,Eckert, D.M.,Laganowsky, A.,Sacchettini, J.,Lindahl, P.,Bankaitis, V.A. A Sec14-like phosphatidylinositol transfer protein paralog defines a novel class of heme-binding proteins. Elife, 9:-, 2020 Cited by PubMed Abstract: Yeast Sfh5 is an unusual member of the Sec14-like phosphatidylinositol transfer protein (PITP) family. Whereas PITPs are defined by their abilities to transfer phosphatidylinositol between membranes in vitro, and to stimulate phosphoinositide signaling in vivo, Sfh5 does not exhibit these activities. Rather, Sfh5 is a redox-active penta-coordinate high spin Fe hemoprotein with an unusual heme-binding arrangement that involves a co-axial tyrosine/histidine coordination strategy and a complex electronic structure connecting the open shell iron -orbitals with three aromatic ring systems. That Sfh5 is not a PITP is supported by demonstrations that heme is not a readily exchangeable ligand, and that phosphatidylinositol-exchange activity is resuscitated in heme binding-deficient Sfh5 mutants. The collective data identify Sfh5 as the prototype of a new class of fungal hemoproteins, and emphasize the versatility of the Sec14-fold as scaffold for translating the binding of chemically distinct ligands to the control of diverse sets of cellular activities. PubMed: 32780017DOI: 10.7554/eLife.57081 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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