6W2M

Abortive ternary complex crystal structure of DNA polymerase Beta with 8OG-dC base pair at the primer terminus and flipped out dA

6W2M の概要

• エントリーDOI: 10.2210/pdb6w2m/pdb
• 分子名称: DNA polymerase beta, Template Strand, Primer Strand, ... (9 entities in total)
• 機能のキーワード: dna polymerase beta, conformational change, enzyme mechanism, transferase, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48667.75

構造登録者

Batra, V.K.,Wilson, S.H. (登録日: 2020-03-06, 公開日: 2020-07-22, 最終更新日: 2023-10-18)

主引用文献

Batra, V.K.,Wilson, S.H.
Structure of a DNA polymerase abortive complex with the 8OG:dA base pair at the primer terminus.
Commun Biol, 3:348-348, 2020

PubMed Abstract: Adenine frequently pairs with the Hoogsteen edge of an oxidized guanine base (8OG) causing G to T transversions. The (syn)8OG:dA base pair is indistinguishable from the cognant base pair and can be extended by DNA polymerases with reduced efficiency. To examine the structural basis of this reduced efficiency, we sought to obtain the structure of the "product" complex of DNA polymerase (pol) β with the (syn)8OG:dA base pair at the primer terminus by soaking the binary complex crystals with a hydrolysable dCTP analogue complementary to the template base G. Crystallographic refinement of the structure revealed that the adenine of the (syn)8OG:dA base pair had been expelled from the primer terminus and a dCMP was inserted opposite 8OG in a reverse orientation; another uninserted molecule of the analogue was bound to the templating base G. This leads to an abortive complex that could form the basis of oxidatively-induced pol β stalling.

PubMed: 32620932
DOI: 10.1038/s42003-020-1080-4

実験手法

X-RAY DIFFRACTION (1.998 Å)