6W01

The 1.9 A Crystal Structure of NSP15 Endoribonuclease from SARS CoV-2 in the Complex with a Citrate

6W01 の概要

• エントリーDOI: 10.2210/pdb6w01/pdb
• 分子名称: Uridylate-specific endoribonuclease, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• 機能のキーワード: sars corona virus, endonuclease, structural genomics, center for structural genomics of infectious diseases, csgid, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85382.58

構造登録者

Kim, Y.,Jedrzejczak, R.,Maltseva, N.,Endres, M.,Godzik, A.,Michalska, K.,Joachimiak, A.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2020-02-28, 公開日: 2020-03-11, 最終更新日: 2023-10-11)

主引用文献

Kim, Y.,Jedrzejczak, R.,Maltseva, N.I.,Wilamowski, M.,Endres, M.,Godzik, A.,Michalska, K.,Joachimiak, A.
Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2.
Protein Sci., 29:1596-1605, 2020

PubMed Abstract: Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading around the world. There is no existing vaccine or proven drug to prevent infections and stop virus proliferation. Although this virus is similar to human and animal SARS-CoVs and Middle East Respiratory Syndrome coronavirus (MERS-CoVs), the detailed information about SARS-CoV-2 proteins structures and functions is urgently needed to rapidly develop effective vaccines, antibodies, and antivirals. We applied high-throughput protein production and structure determination pipeline at the Center for Structural Genomics of Infectious Diseases to produce SARS-CoV-2 proteins and structures. Here we report two high-resolution crystal structures of endoribonuclease Nsp15/NendoU. We compare these structures with previously reported homologs from SARS and MERS coronaviruses.

PubMed: 32304108
DOI: 10.1002/pro.3873

実験手法

X-RAY DIFFRACTION (1.9 Å)