6VY1

Cryo-EM structure of filamentous PFD from Methanocaldococcus jannaschii

Summary for 6VY1

• Entry DOI: 10.2210/pdb6vy1/pdb
• EMDB information: 21455
• Descriptor: Prefoldin subunit alpha 2 (1 entity in total)
• Functional Keywords: helical symmetry, nanowire, prefoldin, protein fibril
• Biological source: Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
• Total number of polymer chains: 14
• Total formula weight: 229748.97

Authors

Wang, F.,Chen, Y.X.,Ing, N.L.,Hochbaum, A.I.,Clark, D.S.,Glover, D.J.,Egelman, E.H. (deposition date: 2020-02-25, release date: 2020-05-13, Last modification date: 2025-05-14)

Primary citation

Chen, Y.X.,Ing, N.L.,Wang, F.,Xu, D.,Sloan, N.B.,Lam, N.T.,Winter, D.L.,Egelman, E.H.,Hochbaum, A.I.,Clark, D.S.,Glover, D.J.
Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires.
Acs Nano, 14:6559-6569, 2020

PubMed Abstract: The transfer of electrons through protein complexes is central to cellular respiration. Exploiting proteins for charge transfer in a controllable fashion has the potential to revolutionize the integration of biological systems and electronic devices. Here we characterize the structure of an ultrastable protein filament and engineer the filament subunits to create electronically conductive nanowires under aqueous conditions. Cryoelectron microscopy was used to resolve the helical structure of gamma-prefoldin, a filamentous protein from a hyperthermophilic archaeon. Conjugation of tetra-heme c3-type cytochromes along the longitudinal axis of the filament created nanowires capable of long-range electron transfer. Electrochemical transport measurements indicated networks of the nanowires capable of conducting current between electrodes at the redox potential of the cytochromes. Functionalization of these highly engineerable nanowires with other molecules, such as redox enzymes, may be useful for bioelectronic applications.

PubMed: 32347705
DOI: 10.1021/acsnano.9b09405

Experimental method

ELECTRON MICROSCOPY (6 Å)