6VWM

70S ribosome bound to HIV frameshifting stem-loop (FSS) and P-site tRNA (non-rotated conformation, Structure I)

This is a non-PDB format compatible entry.

Summary for 6VWM

• Entry DOI: 10.2210/pdb6vwm/pdb
• EMDB information: 21421
• Descriptor: 5S ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (54 entities in total)
• Functional Keywords: hiv fss, frameshifting, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 53
• Total formula weight: 2187196.93

Authors

Loerch, S.,Bao, C.,Ling, C.,Korostelev, A.A.,Grigorieff, N.,Ermolenko, D.M. (deposition date: 2020-02-20, release date: 2020-06-03, Last modification date: 2024-10-30)

Primary citation

Bao, C.,Loerch, S.,Ling, C.,Korostelev, A.A.,Grigorieff, N.,Ermolenko, D.N.
mRNA stem-loops can pause the ribosome by hindering A-site tRNA binding.
Elife, 9:-, 2020

PubMed Abstract: Although the elongating ribosome is an efficient helicase, certain mRNA stem-loop structures are known to impede ribosome movement along mRNA and stimulate programmed ribosome frameshifting via mechanisms that are not well understood. Using biochemical and single-molecule Förster resonance energy transfer (smFRET) experiments, we studied how frameshift-inducing stem-loops from mRNA and the transcript of Human Immunodeficiency Virus (HIV) perturb translation elongation. We find that upon encountering the ribosome, the stem-loops strongly inhibit A-site tRNA binding and ribosome intersubunit rotation that accompanies translation elongation. Electron cryo-microscopy (cryo-EM) reveals that the HIV stem-loop docks into the A site of the ribosome. Our results suggest that mRNA stem-loops can transiently escape the ribosome helicase by binding to the A site. Thus, the stem-loops can modulate gene expression by sterically hindering tRNA binding and inhibiting translation elongation.

PubMed: 32427100
DOI: 10.7554/eLife.55799

Experimental method

ELECTRON MICROSCOPY (3.4 Å)