6VVJ

Cap1G-TPUA

Summary for 6VVJ

• Entry DOI: 10.2210/pdb6vvj/pdb
• NMR Information: BMRB: 30724
• Descriptor: RNA (130-MER) (1 entity in total)
• Functional Keywords: rna, capped rna, viral, mal strain, mal, 5' leader, leader rna, covered cap
• Biological source: Human immunodeficiency virus 1
• Total number of polymer chains: 1
• Total formula weight: 41922.79

Authors

Summers, M.F.,Brown, J.D. (deposition date: 2020-02-18, release date: 2020-04-29, Last modification date: 2024-05-15)

Primary citation

Brown, J.D.,Kharytonchyk, S.,Chaudry, I.,Iyer, A.S.,Carter, H.,Becker, G.,Desai, Y.,Glang, L.,Choi, S.H.,Singh, K.,Lopresti, M.W.,Orellana, M.,Rodriguez, T.,Oboh, U.,Hijji, J.,Ghinger, F.G.,Stewart, K.,Francis, D.,Edwards, B.,Chen, P.,Case, D.A.,Telesnitsky, A.,Summers, M.F.
Structural basis for transcriptional start site control of HIV-1 RNA fate.
Science, 368:413-417, 2020

PubMed Abstract: Heterogeneous transcriptional start site usage by HIV-1 produces 5'-capped RNAs beginning with one, two, or three 5'-guanosines (1G, 2G, or 3G, respectively) that are either selected for packaging as genomes (1G) or retained in cells as translatable messenger RNAs (mRNAs) (2G and 3G). To understand how 5'-guanosine number influences fate, we probed the structures of capped HIV-1 leader RNAs by deuterium-edited nuclear magnetic resonance. The 1G transcript adopts a dimeric multihairpin structure that sequesters the cap, inhibits interactions with eukaryotic translation initiation factor 4E, and resists decapping. The 2G and 3G transcripts adopt an alternate structure with an elongated central helix, exposed splice donor residues, and an accessible cap. Extensive remodeling, achieved at the energetic cost of a G-C base pair, explains how a single 5'-guanosine modifies the function of a ~9-kilobase HIV-1 transcript.

PubMed: 32327595
DOI: 10.1126/science.aaz7959

Experimental method

SOLUTION NMR