6VV5

Cryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike protein

6VV5 の概要

• エントリーDOI: 10.2210/pdb6vv5/pdb
• EMDBエントリー: 21391
• 分子名称: Spike glycoprotein, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: glycoprotein, surface, receptor-binding, membrane fusion, viral protein
• 由来する生物種: Porcine epidemic diarrhea virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 466495.68

構造登録者

Kirchdoerfer, R.N.,Ward, A.B. (登録日: 2020-02-17, 公開日: 2020-02-26, 最終更新日: 2021-04-14)

主引用文献

Kirchdoerfer, R.N.,Bhandari, M.,Martini, O.,Sewall, L.M.,Bangaru, S.,Yoon, K.J.,Ward, A.B.
Structure and immune recognition of the porcine epidemic diarrhea virus spike protein.
Structure, 29:385-392.e5, 2021

PubMed Abstract: Porcine epidemic diarrhea virus (PEDV) is an alphacoronavirus responsible for significant morbidity and mortality in pigs. A key determinant of viral tropism and entry, the PEDV spike protein is a key target for the host antibody response and a good candidate for a protein-based vaccine immunogen. We used electron microscopy to evaluate the PEDV spike structure, as well as pig polyclonal antibody responses to viral infection. The structure of the PEDV spike reveals a configuration similar to that of HuCoV-NL63. Several PEDV protein-protein interfaces are mediated by non-protein components, including a glycan at Asn264 and two bound palmitoleic acid molecules. The polyclonal antibody response to PEDV infection shows a dominance of epitopes in the S1 region. This structural and immune characterization provides insights into coronavirus spike stability determinants and explores the immune landscape of viral spike proteins.

PubMed: 33378641
DOI: 10.1016/j.str.2020.12.003

実験手法

ELECTRON MICROSCOPY (3.5 Å)