6VU1
Cap3G-TAR-F1 is an RNA hairpin. The 1H-1H NOESY data was collected at 308 K in 10 mM KH2PO4 pH 7.4.
Summary for 6VU1
| Entry DOI | 10.2210/pdb6vu1/pdb |
| NMR Information | BMRB: 30723 |
| Descriptor | RNA (34-MER) (1 entity in total) |
| Functional Keywords | rna, virus, capped rna, viral, nucleotides, leader rna, hiv genome, genome, rna genome, start site exposed cap, free cap |
| Biological source | Human immunodeficiency virus 1 |
| Total number of polymer chains | 1 |
| Total formula weight | 11041.56 |
| Authors | Summers, M.F.,Brown, J.D. (deposition date: 2020-02-14, release date: 2020-04-29, Last modification date: 2024-05-15) |
| Primary citation | Brown, J.D.,Kharytonchyk, S.,Chaudry, I.,Iyer, A.S.,Carter, H.,Becker, G.,Desai, Y.,Glang, L.,Choi, S.H.,Singh, K.,Lopresti, M.W.,Orellana, M.,Rodriguez, T.,Oboh, U.,Hijji, J.,Ghinger, F.G.,Stewart, K.,Francis, D.,Edwards, B.,Chen, P.,Case, D.A.,Telesnitsky, A.,Summers, M.F. Structural basis for transcriptional start site control of HIV-1 RNA fate. Science, 368:413-417, 2020 Cited by PubMed Abstract: Heterogeneous transcriptional start site usage by HIV-1 produces 5'-capped RNAs beginning with one, two, or three 5'-guanosines (1G, 2G, or 3G, respectively) that are either selected for packaging as genomes (1G) or retained in cells as translatable messenger RNAs (mRNAs) (2G and 3G). To understand how 5'-guanosine number influences fate, we probed the structures of capped HIV-1 leader RNAs by deuterium-edited nuclear magnetic resonance. The 1G transcript adopts a dimeric multihairpin structure that sequesters the cap, inhibits interactions with eukaryotic translation initiation factor 4E, and resists decapping. The 2G and 3G transcripts adopt an alternate structure with an elongated central helix, exposed splice donor residues, and an accessible cap. Extensive remodeling, achieved at the energetic cost of a G-C base pair, explains how a single 5'-guanosine modifies the function of a ~9-kilobase HIV-1 transcript. PubMed: 32327595DOI: 10.1126/science.aaz7959 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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