6VOC
icosahedral symmetry reconstruction of brome mosaic virus (RNA 3+4)
Summary for 6VOC
| Entry DOI | 10.2210/pdb6voc/pdb |
| EMDB information | 21260 21261 21279 21280 21281 21282 21283 21284 21285 21286 21287 21288 21289 21290 21291 21292 21293 21294 21295 21296 21297 21298 21299 21300 |
| Descriptor | Capsid protein (1 entity in total) |
| Functional Keywords | brome mosaic virus (rna 3 and 4), virus |
| Biological source | Brome mosaic virus (BMV) |
| Total number of polymer chains | 3 |
| Total formula weight | 61234.58 |
| Authors | Beren, C.,Cui, Y.X.,Chakravarty, A.,Yang, X.,Rao, A.L.N.,Knobler, C.M.,Zhou, Z.H.,Gelbart, W.M. (deposition date: 2020-01-30, release date: 2020-05-20, Last modification date: 2025-05-21) |
| Primary citation | Beren, C.,Cui, Y.,Chakravarty, A.,Yang, X.,Rao, A.L.N.,Knobler, C.M.,Zhou, Z.H.,Gelbart, W.M. Genome organization and interaction with capsid protein in a multipartite RNA virus. Proc.Natl.Acad.Sci.USA, 117:10673-10680, 2020 Cited by PubMed Abstract: We report the asymmetric reconstruction of the single-stranded RNA (ssRNA) content in one of the three otherwise identical virions of a multipartite RNA virus, brome mosaic virus (BMV). We exploit a sample consisting exclusively of particles with the same RNA content-specifically, RNAs 3 and 4-assembled in planta by agrobacterium-mediated transient expression. We find that the interior of the particle is nearly empty, with most of the RNA genome situated at the capsid shell. However, this density is disordered in the sense that the RNA is not associated with any particular structure but rather, with an ensemble of secondary/tertiary structures that interact with the capsid protein. Our results illustrate a fundamental difference between the ssRNA organization in the multipartite BMV viral capsid and the monopartite bacteriophages MS2 and Qβ for which a dominant RNA conformation is found inside the assembled viral capsids, with RNA density conserved even at the center of the particle. This can be understood in the context of the differing demands on their respective lifecycles: BMV must package separately each of several different RNA molecules and has been shown to replicate and package them in isolated, membrane-bound, cytoplasmic complexes, whereas the bacteriophages exploit sequence-specific "packaging signals" throughout the viral RNA to package their monopartite genomes. PubMed: 32358197DOI: 10.1073/pnas.1915078117 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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