6VNW

Cryo-EM structure of apo-BBSome

Summary for 6VNW

• Entry DOI: 10.2210/pdb6vnw/pdb
• EMDB information: 21251
• Descriptor: Bardet-Biedl syndrome 18 protein, Bardet-Biedl syndrome 2 protein homolog, Bardet-Biedl syndrome 4 protein homolog, ... (8 entities in total)
• Functional Keywords: protein transport
• Biological source: Bos taurus (Bovine); More
• Total number of polymer chains: 8
• Total formula weight: 486479.94

Authors

Yang, S.,Walz, T.,Nachury, M.,Chou, H. (deposition date: 2020-01-29, release date: 2020-07-01, Last modification date: 2024-03-06)

Primary citation

Yang, S.,Bahl, K.,Chou, H.T.,Woodsmith, J.,Stelzl, U.,Walz, T.,Nachury, M.V.
Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes.
Elife, 9:-, 2020

PubMed Abstract: Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base of cilia. Here, we present the near-atomic structures of the BBSome by itself and in complex with ARL6, and we describe the changes in BBSome conformation induced by ARL6 binding. Modeling the interactions of the BBSome with membranes and the GPCR Smoothened (SMO) reveals that SMO, and likely also other GPCR cargoes, must release their amphipathic helix 8 from the membrane to be recognized by the BBSome.

PubMed: 32510327
DOI: 10.7554/eLife.55954

Experimental method

ELECTRON MICROSCOPY (3.44 Å)