6VLK

A varicella-zoster virus glycoprotein

6VLK の概要

• エントリーDOI: 10.2210/pdb6vlk/pdb
• 分子名称: Envelope glycoprotein B, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: viral membrane glycoprotein, viral protein
• 由来する生物種: Varicella-zoster virus (strain Oka vaccine) (HHV-3)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 173078.84

構造登録者

Xing, Y. (登録日: 2020-01-24, 公開日: 2020-07-15, 最終更新日: 2024-10-16)

主引用文献

Oliver, S.L.,Xing, Y.,Chen, D.H.,Roh, S.H.,Pintilie, G.D.,Bushnell, D.A.,Sommer, M.H.,Yang, E.,Carfi, A.,Chiu, W.,Arvin, A.M.
A glycoprotein B-neutralizing antibody structure at 2.8 angstrom uncovers a critical domain for herpesvirus fusion initiation.
Nat Commun, 11:4141-4141, 2020

PubMed Abstract: Members of the Herpesviridae, including the medically important alphaherpesvirus varicella-zoster virus (VZV), induce fusion of the virion envelope with cell membranes during entry, and between cells to form polykaryocytes in infected tissues. The conserved glycoproteins, gB, gH and gL, are the core functional proteins of the herpesvirus fusion complex. gB serves as the primary fusogen via its fusion loops, but functions for the remaining gB domains remain unexplained. As a pathway for biological discovery of domain function, our approach used structure-based analysis of the viral fusogen together with a neutralizing antibody. We report here a 2.8 Å cryogenic-electron microscopy structure of native gB recovered from VZV-infected cells, in complex with a human monoclonal antibody, 93k. This high-resolution structure guided targeted mutagenesis at the gB-93k interface, providing compelling evidence that a domain spatially distant from the gB fusion loops is critical for herpesvirus fusion, revealing a potential new target for antiviral therapies.

PubMed: 32811830
DOI: 10.1038/s41467-020-17911-0

実験手法

X-RAY DIFFRACTION (2.4529 Å)