6VGS

Alpha-ketoisovalerate decarboxylase (KivD) from Lactococcus lactis, thermostable mutant

これはPDB形式変換不可エントリーです。

6VGS の概要

• エントリーDOI: 10.2210/pdb6vgs/pdb
• 分子名称: Alpha-keto acid decarboxylase, MAGNESIUM ION, THIAMINE DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: thiamine pyrophosphate, lyase
• 由来する生物種: Lactococcus lactis subsp. lactis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 254921.26

構造登録者

Chan, S.,Korman, T.P.,Sawaya, M.R.,Bowie, J.U. (登録日: 2020-01-08, 公開日: 2020-08-05, 最終更新日: 2023-10-11)

主引用文献

Sherkhanov, S.,Korman, T.P.,Chan, S.,Faham, S.,Liu, H.,Sawaya, M.R.,Hsu, W.T.,Vikram, E.,Cheng, T.,Bowie, J.U.
Isobutanol production freed from biological limits using synthetic biochemistry.
Nat Commun, 11:4292-4292, 2020

PubMed Abstract: Cost competitive conversion of biomass-derived sugars into biofuel will require high yields, high volumetric productivities and high titers. Suitable production parameters are hard to achieve in cell-based systems because of the need to maintain life processes. As a result, next-generation biofuel production in engineered microbes has yet to match the stringent cost targets set by petroleum fuels. Removing the constraints imposed by having to maintain cell viability might facilitate improved production metrics. Here, we report a cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity of 4 g L h, a titer of 275 g L and 95% yield over the course of nearly 5 days. These production metrics exceed even the highly developed ethanol fermentation process. Our results suggest that moving beyond cells has the potential to expand what is possible for bio-based chemical production.

PubMed: 32855421
DOI: 10.1038/s41467-020-18124-1

実験手法

X-RAY DIFFRACTION (1.8 Å)