6V4X

Cryo-EM structure of an active human histone pre-mRNA 3'-end processing machinery at 3.2 Angstrom resolution

6V4X の概要

• エントリーDOI: 10.2210/pdb6v4x/pdb
• EMDBエントリー: 21045 21046 21047 21050
• 分子名称: Small nuclear ribonucleoprotein Sm D3, Symplekin, U7 snRNA, ... (13 entities in total)
• 機能のキーワード: endonuclease, active cpsf73, u7 snrnp, 3'-end processing, ribonuclease, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 422255.18

構造登録者

Sun, Y.,Zhang, Y.,Walz, T.,Tong, L. (登録日: 2019-12-02, 公開日: 2020-02-19, 最終更新日: 2025-06-04)

主引用文献

Sun, Y.,Zhang, Y.,Aik, W.S.,Yang, X.C.,Marzluff, W.F.,Walz, T.,Dominski, Z.,Tong, L.
Structure of an active human histone pre-mRNA 3'-end processing machinery.
Science, 367:700-703, 2020

PubMed Abstract: The 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with the canonical cleavage and polyadenylation machinery. We reconstituted an active human histone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its structure by cryo-electron microscopy. The overall structure is highly asymmetrical and resembles an amphora with one long handle. We captured the pre-mRNA in the active site of the endonuclease, the 73-kilodalton subunit of the cleavage and polyadenylation specificity factor, poised for cleavage. The endonuclease and the entire cleavage module undergo extensive rearrangements for activation, triggered through the recognition of the duplex between the authentic pre-mRNA and U7 small nuclear RNA (snRNA). Our study also has notable implications for understanding canonical and snRNA 3'-end processing.

PubMed: 32029631
DOI: 10.1126/science.aaz7758

実験手法

ELECTRON MICROSCOPY (3.2 Å)