6V4U

Cryo-EM structure of SMCR8-C9orf72-WDR41 complex

6V4U の概要

• エントリーDOI: 10.2210/pdb6v4u/pdb
• EMDBエントリー: 21048
• 分子名称: WD repeat-containing protein 41, Guanine nucleotide exchange C9orf72, Guanine nucleotide exchange protein SMCR8 (3 entities in total)
• 機能のキーワード: complex, trimer, autophagy, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 211324.38

構造登録者

Su, M.Y.,Hurley, J.H. (登録日: 2019-12-01, 公開日: 2020-09-02, 最終更新日: 2025-05-14)

主引用文献

Su, M.Y.,Fromm, S.A.,Zoncu, R.,Hurley, J.H.
Structure of the C9orf72 ARF GAP complex that is haploinsufficient in ALS and FTD.
Nature, 585:251-255, 2020

PubMed Abstract: Mutation of C9orf72 is the most prevalent defect associated with amyotrophic lateral sclerosis and frontotemporal degeneration. Together with hexanucleotide-repeat expansion, haploinsufficiency of C9orf72 contributes to neuronal dysfunction. Here we determine the structure of the C9orf72-SMCR8-WDR41 complex by cryo-electron microscopy. C9orf72 and SMCR8 both contain longin and DENN (differentially expressed in normal and neoplastic cells) domains, and WDR41 is a β-propeller protein that binds to SMCR8 such that the whole structure resembles an eye slip hook. Contacts between WDR41 and the DENN domain of SMCR8 drive the lysosomal localization of the complex in conditions of amino acid starvation. The structure suggested that C9orf72-SMCR8 is a GTPase-activating protein (GAP), and we found that C9orf72-SMCR8-WDR41 acts as a GAP for the ARF family of small GTPases. These data shed light on the function of C9orf72 in normal physiology, and in amyotrophic lateral sclerosis and frontotemporal degeneration.

PubMed: 32848248
DOI: 10.1038/s41586-020-2633-x

実験手法

ELECTRON MICROSCOPY (3.8 Å)