6V2C

Complex of mutant (K162M) of E. coli L-asparaginase II with L-Asp. Covalent acyl-enzyme intermediate and tetrahedral intermediate

Summary for 6V2C

Entry DOI	10.2210/pdb6v2c/pdb
Descriptor	L-asparaginase 2 (2 entities in total)
Functional Keywords	l-asparagine hydrolase, anti-cancer drug, catalytically deficient mutant, hydrolase
Biological source	Escherichia coli (strain K12)
Total number of polymer chains	4
Total formula weight	142815.92
Authors	Lubkowski, J.,Wlodawer, A. (deposition date: 2019-11-22, release date: 2020-05-20, Last modification date: 2023-10-11)
Primary citation	Lubkowski, J.,Vanegas, J.,Chan, W.K.,Lorenzi, P.L.,Weinstein, J.N.,Sukharev, S.,Fushman, D.,Rempe, S.,Anishkin, A.,Wlodawer, A. Mechanism of Catalysis by l-Asparaginase. Biochemistry, 59:1927-1945, 2020 Cited by PubMed: 32364696 DOI: 10.1021/acs.biochem.0c00116 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation