6UXW

SWI/SNF nucleosome complex with ADP-BeFx

6UXW の概要

• エントリーDOI: 10.2210/pdb6uxw/pdb
• EMDBエントリー: 20933 20934 20935
• 分子名称: Histone H3.2, Transcription regulatory protein SNF2, SWI/SNF chromatin-remodeling complex subunit SWI1, ... (22 entities in total)
• 機能のキーワード: swi/snf, chromatin remodeler, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 1289692.55

構造登録者

He, Y.,Han, Y. (登録日: 2019-11-08, 公開日: 2020-03-18, 最終更新日: 2025-05-21)

主引用文献

Han, Y.,Reyes, A.A.,Malik, S.,He, Y.
Cryo-EM structure of SWI/SNF complex bound to a nucleosome.
Nature, 579:452-455, 2020

PubMed Abstract: The chromatin-remodelling complex SWI/SNF is highly conserved and has critical roles in various cellular processes, including transcription and DNA-damage repair. It hydrolyses ATP to remodel chromatin structure by sliding and evicting histone octamers, creating DNA regions that become accessible to other essential factors. However, our mechanistic understanding of the remodelling activity is hindered by the lack of a high-resolution structure of complexes from this family. Here we report the cryo-electron microscopy structure of Saccharomyces cerevisiae SWI/SNF bound to a nucleosome, at near-atomic resolution. In the structure, the actin-related protein (Arp) module is sandwiched between the ATPase and the rest of the complex, with the Snf2 helicase-SANT associated (HSA) domain connecting all modules. The body contains an assembly scaffold composed of conserved subunits Snf12 (also known as SMARCD or BAF60), Snf5 (also known as SMARCB1, BAF47 or INI1) and an asymmetric dimer of Swi3 (also known as SMARCC, BAF155 or BAF170). Another conserved subunit, Swi1 (also known as ARID1 or BAF250), resides in the core of SWI/SNF, acting as a molecular hub. We also observed interactions between Snf5 and the histones at the acidic patch, which could serve as an anchor during active DNA translocation. Our structure enables us to map and rationalize a subset of cancer-related mutations in the human SWI/SNF complex and to propose a model for how SWI/SNF recognizes and remodels the +1 nucleosome to generate nucleosome-depleted regions during gene activation.

PubMed: 32188938
DOI: 10.1038/s41586-020-2087-1

実験手法

ELECTRON MICROSCOPY (8.96 Å)