6UXI

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP-Glycine

6UXI の概要

• エントリーDOI: 10.2210/pdb6uxi/pdb
• 分子名称: Serine hydroxymethyltransferase, N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE], 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: folate metabolism, methyltransferase, soybean cyst, nematode infection resistance, cytoplasmic enzyme, plant protein, transferase
• 由来する生物種: Glycine max (Soybean)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104850.72

構造登録者

Korasick, D.A.,Tanner, J.J.,Beamer, L.J. (登録日: 2019-11-07, 公開日: 2020-02-12, 最終更新日: 2023-10-11)

主引用文献

Korasick, D.A.,Kandoth, P.K.,Tanner, J.J.,Mitchum, M.G.,Beamer, L.J.
Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
J.Biol.Chem., 295:3708-3718, 2020

PubMed Abstract: Management of the agricultural pathogen soybean cyst nematode (SCN) relies on the use of SCN-resistant soybean cultivars, a strategy that has been failing in recent years. An underutilized source of resistance in the soybean genotype Peking is linked to two polymorphisms in serine hydroxy-methyltransferase 8 (SHMT8). SHMT is a pyridoxal 5'-phosphate-dependent enzyme that converts l-serine and (6)-tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. Here, we determined five crystal structures of the 1884-residue SHMT8 tetramers from the SCN-susceptible cultivar (cv.) Essex and the SCN-resistant cv. Forrest (whose resistance is derived from the SHMT8 polymorphisms in Peking); the crystal structures were determined in complex with various ligands at 1.4-2.35 Å resolutions. We find that the two Forrest-specific polymorphic substitutions (P130R and N358Y) impact the mobility of a loop near the entrance of the (6)-tetrahydrofolate-binding site. Ligand-binding and kinetic studies indicate severely reduced affinity for folate and dramatically impaired enzyme activity in Forrest SHMT8. These findings imply widespread effects on folate metabolism in soybean cv. Forrest that have implications for combating the widespread increase in virulent SCN.

PubMed: 32014996
DOI: 10.1074/jbc.RA119.012256

実験手法

X-RAY DIFFRACTION (2.1 Å)