6UWG

Engineered variant of I-OnuI meganuclease with improved thermostability and E178D mutation at catalytic site

Summary for 6UWG

Entry DOI	10.2210/pdb6uwg/pdb
Descriptor	I-OnuI-e-Therm-E178D, DNA (26-MER), CALCIUM ION, ... (6 entities in total)
Functional Keywords	meganuclease, homing endonuclease, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
Biological source	synthetic construct More
Total number of polymer chains	3
Total formula weight	51209.41
Authors	Werther, R.,Stoddard, B.L. (deposition date: 2019-11-05, release date: 2019-12-18, Last modification date: 2023-10-11)
Primary citation	Lambert, A.R.,Hallinan, J.P.,Werther, R.,Glow, D.,Stoddard, B.L. Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition. Structure, 28:760-775.e8, 2020 Cited by PubMed: 32359399 DOI: 10.1016/j.str.2020.04.009 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.22 Å)

Structure validation