6UW0

Engineered variant of I-OnuI meganuclease with improved thermostability and fully altered specificity targeting cholera toxin A subunit

6UW0 の概要

• エントリーDOI: 10.2210/pdb6uw0/pdb
• 分子名称: I-OnuI-e-Therm-bCtxA, DNA (27-MER), CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: meganuclease, homing endonuclease, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 101863.70

構造登録者

Werther, R.,Stoddard, B.L. (登録日: 2019-11-04, 公開日: 2019-12-18, 最終更新日: 2023-10-11)

• 主引用文献: Lambert, A.R.,Hallinan, J.P.,Werther, R.,Glow, D.,Stoddard, B.L.; Optimization of Protein Thermostability and Exploitation of Recognition Behavior to Engineer Altered Protein-DNA Recognition.; Structure, 28:760-775.e8, 2020; PubMed: 32359399; DOI: 10.1016/j.str.2020.04.009

実験手法

X-RAY DIFFRACTION (2.72 Å)