6UU9
E. coli mutant sigma-S transcription initiation complex with an 8-nt RNA ("Fresh" mutant crystal soaked with GTP, UTP, CTP, and ddATP for 30 minutes)
This is a non-PDB format compatible entry.
Summary for 6UU9
Entry DOI | 10.2210/pdb6uu9/pdb |
Descriptor | DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, 2',3'-DIDEOXYADENOSINE-5'-MONOPHOSPHATE, ... (12 entities in total) |
Functional Keywords | transcription initiation, rna polymerase, dna promoter, transcription bubble, de novo rna synthesis, dna scrunching, sigma-s factor, transcription, transferase-dna-rna complex, transferase/dna/rna |
Biological source | Escherichia coli More |
Total number of polymer chains | 9 |
Total formula weight | 443050.57 |
Authors | Zuo, Y.,De, S.,Steitz, T.A. (deposition date: 2019-10-30, release date: 2020-08-26, Last modification date: 2023-10-11) |
Primary citation | Zuo, Y.,De, S.,Feng, Y.,Steitz, T.A. Structural Insights into Transcription Initiation from De Novo RNA Synthesis to Transitioning into Elongation. Iscience, 23:101445-101445, 2020 Cited by PubMed: 32829286DOI: 10.1016/j.isci.2020.101445 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (5.4 Å) |
Structure validation
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