6URG

Cryo-EM structure of human CPSF160-WDR33-CPSF30-CPSF100 PIM complex

Summary for 6URG

• Entry DOI: 10.2210/pdb6urg/pdb
• Related: 6URO
• EMDB information: 20859 20860 20861
• Descriptor: Cleavage and polyadenylation specificity factor subunit 1, pre-mRNA 3' end processing protein WDR33, Cleavage and polyadenylation specificity factor subunit 4, ... (5 entities in total)
• Functional Keywords: pre-mrna 3'-end processing, mammalin cleavage factor(mcf), cpsf100, mpsf, protein binding
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 345702.07

Authors

Sun, Y.,Zhang, Y.,Walz, T.,Tong, L. (deposition date: 2019-10-23, release date: 2019-11-27, Last modification date: 2024-03-20)

Primary citation

Zhang, Y.,Sun, Y.,Shi, Y.,Walz, T.,Tong, L.
Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.
Mol.Cell, 77:800-, 2020

PubMed Abstract: The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.

PubMed: 31810758
DOI: 10.1016/j.molcel.2019.11.005

Experimental method

ELECTRON MICROSCOPY (3 Å)