6URG

Cryo-EM structure of human CPSF160-WDR33-CPSF30-CPSF100 PIM complex

6URG の概要

• エントリーDOI: 10.2210/pdb6urg/pdb
• 関連するPDBエントリー: 6URO
• EMDBエントリー: 20859 20860 20861
• 分子名称: Cleavage and polyadenylation specificity factor subunit 1, pre-mRNA 3' end processing protein WDR33, Cleavage and polyadenylation specificity factor subunit 4, ... (5 entities in total)
• 機能のキーワード: pre-mrna 3'-end processing, mammalin cleavage factor(mcf), cpsf100, mpsf, protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 345702.07

構造登録者

Sun, Y.,Zhang, Y.,Walz, T.,Tong, L. (登録日: 2019-10-23, 公開日: 2019-11-27, 最終更新日: 2024-03-20)

主引用文献

Zhang, Y.,Sun, Y.,Shi, Y.,Walz, T.,Tong, L.
Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.
Mol.Cell, 77:800-, 2020

PubMed Abstract: The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.

PubMed: 31810758
DOI: 10.1016/j.molcel.2019.11.005

実験手法

ELECTRON MICROSCOPY (3 Å)