6UQC

Mouse IgG2a Bispecific Fc

6UQC の概要

• エントリーDOI: 10.2210/pdb6uqc/pdb
• 分子名称: Ig gamma-2A chain C region, membrane-bound form, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: bispecific, electrostatic steering, fc, immune system
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 100889.09

構造登録者

Wang, F.,Tsai, J.C.,Davis, J.H.,West, S.M.,Strop, P. (登録日: 2019-10-18, 公開日: 2020-01-01, 最終更新日: 2024-11-13)

主引用文献

Wang, F.,Tsai, J.C.,Davis, J.H.,Chau, B.,Dong, J.,West, S.M.,Hogan, J.M.,Wheeler, M.L.,Bee, C.,Morishige, W.,Cayton, T.,David-Brown, D.,Zhang, C.,Kozhich, A.,Sproul, T.,Dollinger, G.,Rajpal, A.,Strop, P.
Design and characterization of mouse IgG1 and IgG2a bispecific antibodies for use in syngeneic models.
Mabs, 12:1685350-1685350, 2019

PubMed Abstract: The development of antibody therapeutics relies on animal models that accurately recapitulate disease biology. Syngeneic mouse models are increasingly used with new molecules to capture the biology of complex cancers and disease states, and to provide insight into the role of the immune system. The establishment of syngeneic mouse models requires the ability to generate surrogate mouse counterparts to antibodies designed for humans. In the field of bispecific antibodies, there remains a dearth of technologies available to generate native IgG-like mouse bispecific antibodies. Thus, we engineered a simple co-expression system for one-step purification of intact mouse IgG1 and IgG2a bispecific antibodies from any antibody pair. We demonstrated proof of concept with CD3/CD20 bispecific antibodies, which highlighted both the quality and efficacy of materials generated by this technology.

PubMed: 31856660
DOI: 10.1080/19420862.2019.1685350

実験手法

X-RAY DIFFRACTION (1.87 Å)