6UN1

Crystal structure of Pseudomonas aeruginosa PBP3 in complex with temocillin

6UN1 の概要

• エントリーDOI: 10.2210/pdb6un1/pdb
• 関連するPDBエントリー: 6UN3 6UNB
• 分子名称: Peptidoglycan D,D-transpeptidase FtsI, (2R,4S)-2-[(1S)-1-{[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino}-1-methoxy-2-oxoethyl]-5,5-dimethyl-1,3-thiazolidine-4 -carboxylic acid (3 entities in total)
• 機能のキーワード: temocillin, ticarcillin, pseudomonas aeruginosa pbp3, penicillin-binding protein 3, transpeptidase, hmm, high-molecular mass, b-lactam, lactam, penicillin, hydrolase
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58055.19

構造登録者

Sacco, M.,Chen, Y. (登録日: 2019-10-10, 公開日: 2019-10-30, 最終更新日: 2024-10-09)

主引用文献

Sacco, M.D.,Kroeck, K.G.,Kemp, M.T.,Zhang, X.,Andrews, L.D.,Chen, Y.
Influence of the alpha-Methoxy Group on the Reaction of Temocillin with Pseudomonas aeruginosa PBP3 and CTX-M-14 beta-Lactamase.
Antimicrob.Agents Chemother., 64:-, 2019

PubMed Abstract: The prevalence of multidrug-resistant has led to the reexamination of older "forgotten" drugs, such as temocillin, for their ability to combat resistant microbes. Temocillin is the 6-α-methoxy analogue of ticarcillin, a carboxypenicillin with well-characterized antipseudomonal properties. The α-methoxy modification confers resistance to serine β-lactamases, yet temocillin is ineffective against growth. The origins of temocillin's inferior antibacterial properties against have remained relatively unexplored. Here, we analyze the reaction kinetics, protein stability, and binding conformations of temocillin and ticarcillin with penicillin-binding protein 3 (PBP3), an essential PBP in We show that the 6-α-methoxy group perturbs the stability of the PBP3 acyl-enzyme, which manifests in an elevated off-rate constant () in biochemical assays comparing temocillin with ticarcillin. Complex crystal structures with PBP3 reveal similar binding modes of the two drugs but with important differences. Most notably, the 6-α-methoxy group disrupts a high-quality hydrogen bond with a conserved residue important for ligand binding while also being inserted into a crowded active site, possibly destabilizing the active site and enabling water molecule from bulk solvent to access and cleave the acyl-enzyme bond. This hypothesis is supported by the observation that the acyl-enzyme complex of temocillin has reduced thermal stability compared with ticarcillin. Furthermore, we explore temocillin's mechanism of β-lactamase inhibition with a high-resolution complex structure of CTX-M-14 class A serine β-lactamase. The results suggest that the α-methoxy group prevents hydrolysis by locking the compound into an unexpected conformation that impedes access of the catalytic water to the acyl-enzyme adduct.

PubMed: 31685462
DOI: 10.1128/AAC.01473-19

実験手法

X-RAY DIFFRACTION (2.26 Å)