6UM6

Cryo-EM structure of HIV-1 neutralizing antibody DH270.6 in complex with CH848 10.17DT Env

6UM6 の概要

• エントリーDOI: 10.2210/pdb6um6/pdb
• EMDBエントリー: 20818
• 分子名称: CH848 10.17DT gp120, 2-acetamido-2-deoxy-beta-D-glucopyranose, CH848 10.17DT gp41, ... (10 entities in total)
• 機能のキーワード: v3-glycan site, unmutated common ancestor, dh270 lineage, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 368764.46

構造登録者

Acharya, P.,Henderson, R.C.,Saunder, K.O.,Haynes, B.F. (登録日: 2019-10-09, 公開日: 2019-12-18, 最終更新日: 2020-07-29)

主引用文献

Saunders, K.O.,Wiehe, K.,Tian, M.,Acharya, P.,Bradley, T.,Alam, S.M.,Go, E.P.,Scearce, R.,Sutherland, L.,Henderson, R.,Hsu, A.L.,Borgnia, M.J.,Chen, H.,Lu, X.,Wu, N.R.,Watts, B.,Jiang, C.,Easterhoff, D.,Cheng, H.L.,McGovern, K.,Waddicor, P.,Chapdelaine-Williams, A.,Eaton, A.,Zhang, J.,Rountree, W.,Verkoczy, L.,Tomai, M.,Lewis, M.G.,Desaire, H.R.,Edwards, R.J.,Cain, D.W.,Bonsignori, M.,Montefiori, D.,Alt, F.W.,Haynes, B.F.
Targeted selection of HIV-specific antibody mutations by engineering B cell maturation.
Science, 366:-, 2019

PubMed Abstract: A major goal of HIV-1 vaccine development is the design of immunogens that induce broadly neutralizing antibodies (bnAbs). However, vaccination of humans has not resulted in the induction of affinity-matured and potent HIV-1 bnAbs. To devise effective vaccine strategies, we previously determined the maturation pathway of select HIV-1 bnAbs from acute infection through neutralizing antibody development. During their evolution, bnAbs acquire an abundance of improbable amino acid substitutions as a result of nucleotide mutations at variable region sequences rarely targeted by activation-induced cytidine deaminase, the enzyme responsible for antibody mutation. A subset of improbable mutations is essential for broad neutralization activity, and their acquisition represents a key roadblock to bnAb development.

PubMed: 31806786
DOI: 10.1126/science.aay7199

実験手法

ELECTRON MICROSCOPY (4.3 Å)