6UGD
Katanin hexamer in the spiral conformation in complex with substrate
Summary for 6UGD
| Entry DOI | 10.2210/pdb6ugd/pdb |
| EMDB information | 20761 |
| Descriptor | Meiotic spindle formation protein mei-1, Polyglutamate peptide, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total) |
| Functional Keywords | katanin, microtubule-severing, mei-1, microtubule cytoskeleton, motor protein |
| Biological source | Caenorhabditis elegans More |
| Total number of polymer chains | 7 |
| Total formula weight | 326448.24 |
| Authors | Zehr, E.A.,Roll-Mecak, A. (deposition date: 2019-09-26, release date: 2019-10-09, Last modification date: 2024-03-20) |
| Primary citation | Zehr, E.A.,Szyk, A.,Szczesna, E.,Roll-Mecak, A. Katanin Grips the beta-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules. Dev.Cell, 52:118-131.e6, 2020 Cited by PubMed Abstract: The AAA ATPase katanin severs microtubules. It is critical in cell division, centriole biogenesis, and neuronal morphogenesis. Its mutation causes microcephaly. The microtubule templates katanin hexamerization and activates its ATPase. The structural basis for these activities and how they lead to severing is unknown. Here, we show that β-tubulin tails are necessary and sufficient for severing. Cryoelectron microscopy (cryo-EM) structures reveal the essential tubulin tail glutamates gripped by a double spiral of electropositive loops lining the katanin central pore. Each spiral couples allosterically to the ATPase and binds alternating, successive substrate residues, with consecutive residues coordinated by adjacent protomers. This tightly couples tail binding, hexamerization, and ATPase activation. Hexamer structures in different states suggest an ATPase-driven, ratchet-like translocation of the tubulin tail through the pore. A disordered region outside the AAA core anchors katanin to the microtubule while the AAA motor exerts the forces that extract tubulin dimers and sever the microtubule. PubMed: 31735665DOI: 10.1016/j.devcel.2019.10.010 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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