6UEK
Structure of Urocanate Hydratase from Trypanosoma cruzi in complex with NAD+
Summary for 6UEK
| Entry DOI | 10.2210/pdb6uek/pdb |
| Descriptor | Urocanate hydratase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE (3 entities in total) |
| Functional Keywords | chagas disease, urocanate hydratase, nad+, interdomain movement, lyase |
| Biological source | Trypanosoma cruzi |
| Total number of polymer chains | 4 |
| Total formula weight | 305402.01 |
| Authors | Boreiko, S.,Silva, M.,Melo, R.F.P.,Silber, A.M.,Iulek, J. (deposition date: 2019-09-21, release date: 2020-01-15, Last modification date: 2023-10-11) |
| Primary citation | Boreiko, S.,Silva, M.,de F P Melo, R.,Silber, A.M.,Iulek, J. Structure of Urocanate Hydratase from the protozoan Trypanosoma cruzi. Int.J.Biol.Macromol., 146:716-724, 2019 Cited by PubMed Abstract: The enzyme Urocanate Hydratase (UH) participates in the catabolic pathway of L-histidine. Trypanosoma cruzi Urocanate Hydratase (TcUH) is identified as a therapeutic molecular target in the WHO/TDR Targets Database. We report the 3D structure determination and number of features of TcUH, and compared it to other few available bacterial UH structures. Each monomer presents two domains and one NAD molecule. Superpositions revealed differences in the relative orientation of domains within monomers, such that TcUH monomer A resembles Urocanate Hydratase from Geobacillus kaustophilus (GkUH) (open conformation), while monomer C resembles Urocanate Hydratase from Pseudomonas putida (PpUH) and Urocanate Hydratase from Bacillus subtilis (BsUH) (closed conformations). We use the structure of TcUH to make considerations about 3 non-deleterious and 2 deleterious mutations found in human UHs: non-deleterious mutations could be accommodated without large displacements or interaction interruptions, whereas deleterious mutations in one case might disrupt an α-helix (as previously suggested) and in the other case, besides disrupting the enzyme interaction with the substrate, might interfere with interdomain movement. PubMed: 31843618DOI: 10.1016/j.ijbiomac.2019.12.101 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.16 Å) |
Structure validation
Download full validation report
