6U7V
xRRM structure of spPof8
Summary for 6U7V
| Entry DOI | 10.2210/pdb6u7v/pdb |
| Descriptor | Protein pof8, NITRATE ION (3 entities in total) |
| Functional Keywords | telomere, telomerase, rna binding, gene regulation, ter1, xrrm |
| Biological source | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Total number of polymer chains | 1 |
| Total formula weight | 15551.24 |
| Authors | Kim, J.-K.,Hu, X.,Yu, C.,Jun, H.-I.,Liu, J.,Sankaran, B.,Huang, L.,Qiao, F. (deposition date: 2019-09-03, release date: 2020-09-09, Last modification date: 2024-10-30) |
| Primary citation | Hu, X.,Kim, J.K.,Yu, C.,Jun, H.I.,Liu, J.,Sankaran, B.,Huang, L.,Qiao, F. Quality-Control Mechanism for Telomerase RNA Folding in the Cell. Cell Rep, 33:108568-108568, 2020 Cited by PubMed Abstract: Long non-coding RNAs can often fold into different conformations. Telomerase RNA, an essential component of the telomerase ribonucleoprotein (RNP) enzyme, must fold into a defined structure to fulfill its function with the protein catalytic subunit (TERT) and other accessory factors. However, the mechanism by which the correct folding of telomerase RNA is warranted in a cell is still unknown. Here we show that La-related protein Pof8 specifically recognizes the conserved pseudoknot region of telomerase RNA and instructs the binding of the Lsm2-8 complex to its mature 3' end, thus selectively protecting the correctly folded RNA from exonucleolytic degradation. In the absence of Pof8, TERT assembles with misfolded RNA and produces little telomerase activity. Therefore, Pof8 plays a key role in telomerase RNA folding quality control, ensuring that TERT only assembles with functional telomerase RNA to form active telomerase. Our finding reveals a mechanism for non-coding RNA folding quality control. PubMed: 33378677DOI: 10.1016/j.celrep.2020.108568 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.42 Å) |
Structure validation
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