6U5B
CryoEM Structure of Pyocin R2 - precontracted - baseplate
This is a non-PDB format compatible entry.
Summary for 6U5B
| Entry DOI | 10.2210/pdb6u5b/pdb |
| Related | 6PYT |
| EMDB information | 20526 20643 |
| Descriptor | Tri1a PA0618, Sheath PA0622, Tube PA0623, ... (7 entities in total) |
| Functional Keywords | bacteriocin, pyocin, viral protein, antimicrobial protein |
| Biological source | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) More |
| Total number of polymer chains | 60 |
| Total formula weight | 1518427.72 |
| Authors | Ge, P.,Avaylon, J.,Scholl, D.,Shneider, M.M.,Browning, C.,Buth, S.A.,Plattner, M.,Ding, K.,Leiman, P.G.,Miller, J.F.,Zhou, Z.H. (deposition date: 2019-08-27, release date: 2020-04-15, Last modification date: 2024-03-20) |
| Primary citation | Ge, P.,Scholl, D.,Prokhorov, N.S.,Avaylon, J.,Shneider, M.M.,Browning, C.,Buth, S.A.,Plattner, M.,Chakraborty, U.,Ding, K.,Leiman, P.G.,Miller, J.F.,Zhou, Z.H. Action of a minimal contractile bactericidal nanomachine. Nature, 580:658-662, 2020 Cited by PubMed Abstract: R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics. Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold. Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage. Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes; the dissociation then initiates a cascade of events leading to sheath contraction; and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium. PubMed: 32350467DOI: 10.1038/s41586-020-2186-z PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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