6U2M

Crystal structure of a HaloTag-based calcium indicator, HaloCaMP V2, bound to JF635

Summary for 6U2M

• Entry DOI: 10.2210/pdb6u2m/pdb
• Descriptor: HaloCaMP V2, (1E,3S)-1-{10-[2-carboxy-5-({2-[2-(hexyloxy)ethoxy]ethyl}carbamoyl)phenyl]-7-(3-fluoroazetidin-1-yl)-5,5-dimethyldibenz o[b,e]silin-3(5H)-ylidene}-3-fluoroazetidin-1-ium, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: halotag, calcium, sensor, fluorescent, hydrolase
• Biological source: Rhodococcus sp.
• Total number of polymer chains: 2
• Total formula weight: 113499.40

Authors

Deo, C.,Schreiter, E.R. (deposition date: 2019-08-20, release date: 2020-09-30, Last modification date: 2023-10-11)

• Primary citation: Deo, C.,Abdelfattah, A.S.,Bhargava, H.K.,Berro, A.J.,Falco, N.,Farrants, H.,Moeyaert, B.,Chupanova, M.,Lavis, L.D.,Schreiter, E.R.; The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.; Nat.Chem.Biol., 17:718-723, 2021; PubMed: 33795886; DOI: 10.1038/s41589-021-00775-w

Experimental method

X-RAY DIFFRACTION (2 Å)