6TX2

Human HPF1

6TX2 の概要

• エントリーDOI: 10.2210/pdb6tx2/pdb
• 分子名称: Histone PARylation factor 1, SODIUM ION (3 entities in total)
• 機能のキーワード: parp1, parp2, adp-ribosylation, serine adp-ribosylation, c4orf27, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36776.02

構造登録者

Suskiewicz, M.J.,Ahel, I. (登録日: 2020-01-13, 公開日: 2020-02-19, 最終更新日: 2024-05-01)

主引用文献

Suskiewicz, M.J.,Zobel, F.,Ogden, T.E.H.,Fontana, P.,Ariza, A.,Yang, J.C.,Zhu, K.,Bracken, L.,Hawthorne, W.J.,Ahel, D.,Neuhaus, D.,Ahel, I.
HPF1 completes the PARP active site for DNA damage-induced ADP-ribosylation.
Nature, 579:598-602, 2020

PubMed Abstract: The anti-cancer drug target poly(ADP-ribose) polymerase 1 (PARP1) and its close homologue, PARP2, are early responders to DNA damage in human cells. After binding to genomic lesions, these enzymes use NAD to modify numerous proteins with mono- and poly(ADP-ribose) signals that are important for the subsequent decompaction of chromatin and the recruitment of repair factors. These post-translational modifications are predominantly serine-linked and require the accessory factor HPF1, which is specific for the DNA damage response and switches the amino acid specificity of PARP1 and PARP2 from aspartate or glutamate to serine residues. Here we report a co-structure of HPF1 bound to the catalytic domain of PARP2 that, in combination with NMR and biochemical data, reveals a composite active site formed by residues from HPF1 and PARP1 or PARP2 . The assembly of this catalytic centre is essential for the addition of ADP-ribose moieties after DNA damage in human cells. In response to DNA damage and occupancy of the NAD-binding site, the interaction of HPF1 with PARP1 or PARP2 is enhanced by allosteric networks that operate within the PARP proteins, providing an additional level of regulation in the induction of the DNA damage response. As HPF1 forms a joint active site with PARP1 or PARP2, our data implicate HPF1 as an important determinant of the response to clinical PARP inhibitors.

PubMed: 32028527
DOI: 10.1038/s41586-020-2013-6

実験手法

X-RAY DIFFRACTION (2.09 Å)