6TVR
Crystal structure of the haemagglutinin mutant (Gln226Leu) from an H10N7 seal influenza virus isolated in Germany
Summary for 6TVR
| Entry DOI | 10.2210/pdb6tvr/pdb |
| Descriptor | Hemagglutinin HA1, Hemagglutinin HA2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
| Functional Keywords | receptor binding, fusion of virus membrane with host plasma membrane, viral protein |
| Biological source | Influenza A virus (A/harbour seal/Germany/1/2014(H10N7)) More |
| Total number of polymer chains | 12 |
| Total formula weight | 336962.21 |
| Authors | Zhang, J.,Xiong, X.,Purkiss, A.,Walker, P.,Gamblin, S.,Skehel, J.J. (deposition date: 2020-01-10, release date: 2020-10-21, Last modification date: 2024-11-06) |
| Primary citation | Herfst, S.,Zhang, J.,Richard, M.,McBride, R.,Lexmond, P.,Bestebroer, T.M.,Spronken, M.I.J.,de Meulder, D.,van den Brand, J.M.,Rosu, M.E.,Martin, S.R.,Gamblin, S.J.,Xiong, X.,Peng, W.,Bodewes, R.,van der Vries, E.,Osterhaus, A.D.M.E.,Paulson, J.C.,Skehel, J.J.,Fouchier, R.A.M. Hemagglutinin Traits Determine Transmission of Avian A/H10N7 Influenza Virus between Mammals. Cell Host Microbe, 28:602-613.e7, 2020 Cited by PubMed Abstract: In 2014, an outbreak of avian A/H10N7 influenza virus occurred among seals along North-European coastal waters, significantly impacting seal populations. Here, we examine the cross-species transmission and mammalian adaptation of this influenza A virus, revealing changes in the hemagglutinin surface protein that increase stability and receptor binding. The seal A/H10N7 virus was aerosol or respiratory droplet transmissible between ferrets. Compared with avian H10 hemagglutinin, seal H10 hemagglutinin showed stronger binding to the human-type sialic acid receptor, with preferential binding to α2,6-linked sialic acids on long extended branches. In X-ray structures, changes in the 220-loop of the receptor-binding pocket caused similar interactions with human receptor as seen for pandemic strains. Two substitutions made seal H10 hemagglutinin more stable than avian H10 hemagglutinin and similar to human hemagglutinin. Consequently, identification of avian-origin influenza viruses across mammals appears critical to detect influenza A viruses posing a major threat to humans and other mammals. PubMed: 33031770DOI: 10.1016/j.chom.2020.08.011 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.63 Å) |
Structure validation
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