6TUT

Cryo-EM structure of the RNA Polymerase III-Maf1 complex

6TUT の概要

• エントリーDOI: 10.2210/pdb6tut/pdb
• EMDBエントリー: 10595
• 分子名称: DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (19 entities in total)
• 機能のキーワード: transcription, rna polymerase iii, pol iii, maf1, transcription inhibition
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 725061.82

構造登録者

Vorlaender, M.K.,Hagen, W.J.H.,Mueller, C.W. (登録日: 2020-01-08, 公開日: 2020-02-19, 最終更新日: 2024-05-22)

主引用文献

Vorlander, M.K.,Baudin, F.,Moir, R.D.,Wetzel, R.,Hagen, W.J.H.,Willis, I.M.,Muller, C.W.
Structural basis for RNA polymerase III transcription repression by Maf1.
Nat.Struct.Mol.Biol., 27:229-232, 2020

PubMed Abstract: Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex.

PubMed: 32066962
DOI: 10.1038/s41594-020-0383-y

実験手法

ELECTRON MICROSCOPY (3.25 Å)